Mid morning, after the lights have been on for a couple of hours and any condensation has dried, is the best window for applying pollen. Damp pistils and wet pollen clump together and the grains burst before they can grow a tube, so pollinating right after a watering or when humidity has spiked wastes the effort. Practical timing notes, including waiting a day after any foliar spray, are added on https://breederregularseeds.com. I also hold off misting the room for a full day afterward.